Generation of Genomic Mini-Libraries by Taq DNA Polymerase Modification of Genomic Fragments
نویسندگان
چکیده
منابع مشابه
Generation of genomic mini-libraries by Taq DNA polymerase modification of genomic fragments.
When cloning genomic DNA fragments, the efficiency is greatly reduced as the size of the restriction fragment increases. Libraries derived from the total genome are therefore biased because shorter fragments will be cloned preferentially. When cloning a particular gene, it is most desirable to determine a restriction enzyme that will generate a fragment of a reasonable size for the gene of inte...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1998
ISSN: 0736-6205,1940-9818
DOI: 10.2144/98244bm09